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KMID : 0644319980040010017
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Journal of Korean Oriental Oncology
1998 Volume.4 No. 1 p.17 ~ p.36
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Study on the Effect of Hwallakhyoreungdan(活絡效靈丹) on Angiogenic Inhibition Mechanism
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羅琪煥/Na, Ki-Whan
崔昇勳/安圭錫/Choi, Seung-Hoon/Ahn, Kyoo-Seok
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Abstract
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This experimental study was carried out to evaluate the effects of Hwallakhyoreungdan on angiogenic inhibition mechanism. In order to investigate the effects of Hwallakhyoreungdan on angiogenic inhibition mechanism, MTT assay, cell adhesive inhibition effect, DNA fragmentation analysis, Nuclear condensation assay, FACScan analysis. Angiogenic lumen formation assay. Immunocytochemistry analysis, RT-PCR for mRNA expression, Western blot analysis and Confocal analysis for Ca^2+ change were performed
The results were summarized as follows:
1. The cell adhesive inhibition ability was strong from 5㎍/㎖.
2. The G_0/G_1 arresr peak was existed on ECV304 cell-line.
3. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by HR water extract.
4. Angiogenic human formation was inhibited by HR water extract.
5. LFA-1 and ELAM-1´s expression were inhibited by HR water extract. THey are commonly participation on inflammation and tumor regeneration.
6. The expression of MMP-9 and uPA were inhibited by HR water extract.
7. The expression of integrin α_vβ_3 was inhibited by HR water extract.
8. The expression of intracellular molecule were successively inhibited by HR water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced.
9. The change of Ca^2+ was decreased by HR water extract it cause confusion of signal transduction pathway therefore it was take part in apaptosis.
According to the results, Hwallakhyoreungdan showed to be a key antagonist of integrin α_vβ_3, and to be induction of apoptosis by p53 through flow cytornetry. This report also demonstrated that expressions of MMP-9 and uPA was blocked under the angiogenesis model. Thus, we suggests that Hwallakhyoreungdan blocks angiogenesis by inducing apoptosis of ECV304 and ECVPAR cell lines and another oriental herbal medicine that treats blood-stasis type also has angiogenic inhibition effects.
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KEYWORD
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